Structural, biosynthetic and serological cross-reactive elucidation of capsular polysaccharides from Streptococcus pneumoniae serogroup 28
نویسندگان
چکیده
• Structural elucidation of two pneumococcal capsular polysaccharides (CPS) Comparison CPS structures and biosynthesis between serotype 28 F, 28A 16 F Correlation with supported proposed function glycosyltransferase Latex agglutination revealed the importance O Ac PO 4 in serological reaction Capsular are key virulent factors pathogenesis Streptococcus pneumoniae . The previously unknown 28F were thoroughly characterized by NMR spectroscopy, chemical analysis AF4-MALS-dRI. following repeat unit determined: -4)[?- l -Rhap-[4-P-2-Gro]]-(1-3)-?- d -Sug-[6-P-Cho]-(1-3)-?- -Rhap-[2-OAc]-(1-4)-?- -Glcp-(1-; 28F: Sug = Glc p , Mw: 540.5 kDa; 28A: NAc, 421.9 correlation showed that WciU serotypes had different sugar donor specificity toward ? -d -Glc -GlcNAc respectively. Furthermore, latex tests de- de-PO conducted to understand cross-reactions serogroup factor antiserum 23d. Interestingly, can still weakly react 23d, while did not This indicated group could affect affinity P-2-Gro was crucial for cross-reacting
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ژورنال
عنوان ژورنال: Carbohydrate Polymers
سال: 2021
ISSN: ['1879-1344', '0144-8617']
DOI: https://doi.org/10.1016/j.carbpol.2020.117323